The billboard picturing Melania Trump about to make a speech in front of the American flag was accompanied with the phrase, "Just imagine how far you can go with a little bit of English".
It was part of a marketing campaign by private English language school Americki Institut, which tried to persuade Croats to learn English by reminding them of the Slovenian-born US first lady's personal experience.
But Mrs Trump did not accept what was apparently meant to be a joke.
Her Slovenian lawyer demanded that the billboards be immediately removed.
Cars drive in front of a huge advert poster of the 'American Institute' promoting its English language courses with a photo of US First Lady Melania Trump. Source: AAP
Lawyer Natasa Pirc-Musar said she is "satisfied with the fact that the school admitted that they violated the law" and that the billboards were removed on Tuesday.
She says, "we are still analysing possible further legal steps."
Since the billboard was removed Americki Institut uploaded a video of the song, "I'm Sorry" by 1960s country singer Brenda Lee, to their .
Melania Trump was born in neighbouring Slovenia in Melanija Knavs. She left in her 20s to pursue a modelling career in the United States.
Cars drive past the empty advertising space from which a billboard depicting the first lady Melania Trump was removed, in Zagreb, Croatia, Tuesday, Sept. 19. Source: AAP
